The effect of scanning pathways on trueness and precision in full-arch optical impression.

BACKGROUND: In this study, we investigated the effects of differences in scanning pathways during optical impression on the trueness and precision of full-arch impressions. METHODS: Reference data were obtained using a laboratory scanner. All optical impressions were measured across the dental arch using TRIOS® 3 in four different pathways. The reference and optical impression data were superimposed using the best-fit method. The criteria for superimposition were based on the starting side of the dental arch (partial arch best-fit method, PB) and based on the full arch (full arch best-fit method, FB). The data were compared between the left and right molars (starting and ending sides). The scan deviations for trueness (n = 5) and precision (n = 10) were obtained for each group by calculating the root mean square (RMS) of the deviation at each measurement point. Visual observations using superimposed color map images revealed variations in trueness. RESULTS: There were no significant differences in scanning time or amount of scan data between the four scanning pathways. Trueness did not differ significantly among the four pathways with respect to the starting and ending sides, regardless of the superimposition criteria. Precision with PB was significantly different between scanning pathways A and B, and pathways B and C for the starting sides, and between scanning pathways A and B, and pathways A and D for the ending sides. In contrast, there was no significant difference between the starting and ending side in pathways for FB. Regarding PB, color map images showed a large error range in the direction toward the molar radius for the occlusal surface and cervical regions on the ending sides. CONCLUSION: Differences in the scanning pathways did not affect trueness, regardless of the superimposition criteria. On the other hand, differences in the scanning pathways affected the precision of the starting and ending sides with PB. Scanning pathways B and D were more precise on the starting and ending sides, respectively.

Effects of Cuspal Inclination and Luting Agent on Fracture Load Values in Composite Resin CAD/CAM Crowns.

The purpose of this study was to evaluate whether change in cuspal inclination influences the fracture load values of composite resin computer-aided design/computer-aided manufacturing (CAD/CAM) crowns. Abutment teeth and CAD/CAM crowns were prepared as they would be for treating a mandibular first premolar with two cusps. The CAD/CAM crowns were designed so that 1) the principal stress lines would be radially distributed from the two points of contact with the indenter to the occlusal area of the abutment (Type I), or 2) the principal stress lines would pass outside the occlusal area of the abutment (Types II and III). The CAD/CAM crowns were mounted on the abutments using one of two types of resin or polycarboxylate cement. Fracture load values were measured using a universal tester. The Type I CAD/CAM crowns exhibited the highest mean fracture load value, followed by the Type II crowns and then the Type III crowns, with significant differences seen between all types (p<0.05). The luting agent used (resin or polycarboxylate cement) showed no effect on the fracture load value. With the Type II and Type III crowns, significant differences in the fracture load value were observed between the Super-Bond and Hy-Bond polycarboxylate cement groups (p<0.05).

Sintering distortion of monolithic zirconia in 4-unit fixed partial denture: Effect of layered structure and vertical milling area.

Layered-type zirconia disks, which offer color gradation from enamel to cervical shade, have been employed in recent years to replicate the shades of natural teeth. The layered structure is effective at replicating colors and has helped popularize monolithic zirconia restorations. However, the sintering shrinkage of zirconia is very large; thus, controlling the sintering distortion is very important. Thus, the objective of this study was to determine the influence exerted by the layered structure of the zirconia disk and the vertical milling area on the sintering distortion. An experimental fixed partial denture (FPD) was designed based on a 4-unit monolithic zirconia FPD. A single-composition (SC)-type disk with no shade and a single-composition-layered (SCL)-type disk with shade gradation were selected for this study. In particular, three milling areas, the top end of the disk (area I), vertical center (area II), and bottom end of the disk (area III), were investigated. Moreover, the sintering distortions generated by the experimental FPDs were measured. Results showed that sintering distortion in 4-unit monolithic zirconia FPDs occurred in all SC and SCL areas. Additionally, the sintering distortions were affected by the layered structure of the zirconia disks, the degree of which depended on the milling area (area I > area II > area III). Thus, when fabricating dental prosthesis using SCL zirconia disks, the milling area must be selected considering both the color adjustment and sintering distortion.

Influence of one-wall remaining coronal tooth with resin abutment and fiber post on static and dynamic fracture resistance.

The objective of this study was to determine the influence of height and thickness of the one wall remaining coronal tooth structure on the fracture resistance of an endodontically treated root with resin abutment build-up using resin composite and fiber-reinforced resin composite post. Static and dynamic fracture tests were performed by placing the remaining tooth wall on the tensile side and applying loads at an angle of 30° from the tooth axis. Superior static fracture resistance was observed when the wall remaining on the tooth had a height and thickness greater than 1.0 mm. The dynamic fatigue test showed high loading capacity or fracture resistance in specimens with large height and thickness. The dynamic fatigue test showed the influence of the remaining tooth structure on fracture resistance clearly. In conclusion, the static and dynamic fracture resistances increased with the height and thickness of the one wall remaining tooth structure.

Muscle-bone relationship in temporomandibular joint disorders after partial discectomy.

OBJECTIVES: Temporomandibular joint osteoarthritis (TMJ-OA) causes degenerative changes in TMJ tissues. The inter-tissue crosstalk that exacerbates illness and organic changes in bone secondary to TMJ-OA potentially affects the muscles; therefore, patients with a muscular disease might also suffer from bone disease. However, knowledge gaps exist concerning muscle pathology at the onset of TMJ-OA. In this study, we documented the pathogeneses of the bone and muscle at the onset of TMJ-OA using a mouse model. METHODS: We performed a partial resection of the TMJ disk to establish a mouse model of TMJ-OA. After the onset of TMJ-OA, we performed various measurements at 8, 12, and 16 weeks post-surgery in the defined groups. RESULTS: The volume of the mandibular head in the TMJ-OA group was significantly greater than that in the control group. The temporal muscles in the TMJ-OA group were significantly deformed compared with those in the control group; however, between-group comparisons did not reveal significant differences in the mandibular head or temporal muscles after surgery. Therefore, we hypothesized that the degree of mandibular head hypertrophy would alter the temporal muscles. A subsequent analysis of the correlation between the bone and muscle confirmed that the deformity of the temporal muscle increased with increasing hypertrophy of the mandibular head. Temporal and masseter muscle contact was observed in 25% of surgical groups. CONCLUSIONS: This study demonstrates that TMJ-OA progressed when organic changes occurred in bones and muscles, supporting the symbiotic relationship between bones and muscles.

Implant and Prosthetic Treatment in Esthetic Zone with Alveolar Ridge Preservation and Autotransplantation: Clinical Case Report with 16-year Follow-up.

Occlusal reconstruction comprising orthodontic treatment, autotransplantation, and implant treatment was performed in a 30-year-old woman with missing maxillary anterior teeth. An initial examination revealed marked root resorption of the maxillary anterior teeth. Both the maxillary canines were missing, causing constriction of the dental arch. Conserving the right maxillary central and lateral incisors, which were dislocated due to trauma, was considered to be too difficult, and a bridge was not selected as there was insufficient load bearing capacity in the adjacent abutment teeth, making the prognosis uncertain. Partial dentures were rejected due to the patient's age and esthetic demands. First, the right mandibular lateral incisor was extracted to relieve crowding in the mandibular anterior teeth, leaving the patient with 3 mandibular incisors. Orthodontic treatment was then performed to harmonize the occlusal relationship between the mandibular and maxillary anterior teeth. Next, the right mandibular lateral incisor was transplanted to the extraction site of the right maxillary lateral incisor. After fixation of the autotransplanted tooth, the shape of the bone around the implant site improved. The final prosthesis for the right maxillary central incisor was provided via implant treatment. As seen in a follow-up 16 years later, the interproximal dental papilla was intact, and the patient's esthetic demands were fulfilled. Utilizing the functions of the periodontal ligament of the natural teeth was a useful part of orthodontic treatment and autotransplantation.

Plaque-removing Effects of Interdental Instruments in Molar Region.

Many types of interdental instrument, such as dental floss and interdental brushes, can be purchased easily and sell in large numbers. Many studies have compared the effects of such instruments. Few studies have investigated their relationship with regions of residual plaque, however. The purpose of this study was to compare rates of plaque removal from the mesial and distal surfaces of the bilateral maxillary and mandibular premolars and molars among 3 types of interdental instrument: finger-winding-dental floss, holder-type dental floss, and an interdental brush. Prior to the experiment, the artificial teeth in a jaw model were removed for application of artificial plaque. The teeth were then replaced and the model attached to a phantom. An operator brushed the teeth in a posture close to that adopted in actual tooth brushing with each type of instrument, after which the plaque removal rate was compared among them. The rate of plaque removal using finger-winding floss was the highest, although this type of instrument is less easy to hold than the others. The rate with a handle-equipped interdental instrument showed a decrease in some regions, although it is easy to hold. The results of this study suggest that the type of interdental instrument used should differ depending on the region to be cleaned.

Temporomandibular Joint Hypofunction Secondary to Unilateral Partial Discectomy Attenuates Degeneration in Murine Mandibular Condylar Cartilage.

Mechanical overloading of the temporomandibular joint (TMJ) promotes both the initiation and progression of TMJ osteoarthritis (OA). New preclinical animal models are needed for the evaluation of the molecular basis of cellular load transmission. This would allow a better understanding of the underlying mechanisms of TMJ-OA pain and disability, and help identify new therapeutics for its early diagnosis and management. The purpose of this study was to evaluate the role of mechanical loading in the progression of TMJ-OA in surgical instability arising from unilateral partial discectomy (UPD) in a murine model. In the theoretical modelling employed, lower joint reaction forces were observed on the chewing (working) side of the TMJ in the murine craniomandibular musculoskeletal system. Hypofunction was induced secondary to UPD through surgically manipulating the working side using an unopposed molar model. When the working side was restricted to the same side as that on which UPD was performed, late-stage degeneration of the cartilage showed a significant reduction (p<0.05), with diminished fibrillation and erosion of the articular cartilage, cell clustering, and hypocellularity. Condylar remodelling and proteolysis of proteoglycans were less affected. Thus, select and specific late-stage changes in TMJ-OA were contextually linked with the local mechanical environment of the joint. These data underscore the value of the UPD mouse model in studying mechanobiological pathways activated during TMJ-OA, and suggest that therapeutically targeting mechanobiological stimuli is an effective strategy in improving long-term biological, clinical, and patient-based outcomes.

Two-photon fluorescence and second harmonic generation characterization of extracellular matrix remodeling in post-injury murine temporomandibular joint osteoarthritis.

End stage temporomandibular joint osteoarthritis (TMJ-OA) is characterized by fibrillations, fissures, clefts, and erosion of the mandibular condylar cartilage. The goal of this study was to define changes in pericellular and interterritorial delineations of the extracellular matrix (ECM) that occur preceding and concurrent with the development of this end stage degeneration in a murine surgical instability model. Two-photon fluorescence (TPF) and second harmonic generation (SHG) microscopy was used to evaluate TMJ-OA mediated changes in the ECM. We illustrate that TPF/SHG microscopy reconstructs the three-dimensional network of key fibrillar and micro-fibrillar collagens altered during the progression of TMJ-OA. This method not only generates spatially distinct pericellular and interterritorial delineations of the ECM but distinguishes early and end stage TMJ-OA by signal organization, orientation, and composition. Early stage TMJ-OA at 4- and 8-weeks post-injury is characterized by two structurally distinct regions containing dense, large fiber collagens and superficial, small fiber collagens rich in types I, III, and VI collagen oriented along the mesiodistal axis of the condyle. At 8-weeks post-injury, type VI collagen is locally diminished on the central and medial condyle, but the type I/III rich superficial layer is still present. Twelve- and 16-weeks post-injury mandibular cartilage is characteristic of end-stage disease, with hypocellularity and fibrillations, fissures, and clefts in the articular layer that propagate along the mediolateral axis of the MCC. We hypothesize that the localized depletion of interterritorial and pericellular type VI collagen may signify an early marker for the transition from early to end stage TMJ-OA, influence the injury response of the tissue, and underlie patterns of degeneration that follow attritional modes of failure.

Neuron/Glial Antigen 2-Type VI Collagen Interactions During Murine Temporomandibular Joint Osteoarthritis.

The degeneration of articular cartilage underscores the clinical pathology of temporomandibular joint osteoarthritis (TMJ-OA) and is promoted through dysfunctional biochemical or biophysical signaling. Transduction of these signals has a multifaceted regulation that includes important cell-matrix derived interactions. The matrix encapsulating the cells of the mandibular condylar cartilage (MCC) is rich in type VI collagen. Neuron/glia antigen 2 (NG2) is a type I transmembrane proteoglycan that binds with type VI collagen. This study defines the temporospatial dynamics of NG2-type VI collagen interactions during the progression of TMJ-OA. Membrane-bound NG2 is found to colocalize with pericellular type VI collagen in superficial layer cells in the MCC perichondrium but is present at high levels in the cytosol of chondroblastic and hypertrophic cells. When TMJ -OA is induced using a surgical instability model, localized disruptions of pericellular type VI collagen are observed on the central and medial MCC and are associated with significantly higher levels of cytosolic NG2. NG2 localized within the cytosol is found to be transported through clathrin and dynamin mediated endocytic pathways. These findings are consistent with NG2 behavior in other injury models and underscore the potential of NG2 as an entirely novel molecular mechanism of chondrocyte function contextually linked with TMJ-OA.

Effect of occlusal groove on abutment, crown thickness, and cement-type on fracture load of monolithic zirconia crowns.

The aim of this study was to investigate the effects of occlusal form of abutment, occlusal thickness of monolithic zirconia crowns (MZC), and cement type on the fracture load of MZC. Abutments were prepared with 2 types of occlusal forms: groove-type and flat-type. These were designed so that thickness at the central fissure region of MZC was 0.3, 0.5, or 0.7 mm. Glass ionomer cement and resin cement were used to lute MZC to their corresponding abutment. Fracture load was determined using a universal testing machine. As a result, groove-type abutment had lower fracture load compared to flat-type abutment; however, the decline in strength was smaller when resin cement was used. Additionally, specimens with larger occlusal thickness had greater fracture load regardless of groove or cement-type. The fracture of MZC occurred on the central fissure region of MZC except for 0.7 mm groove-type MZC luted with resin cement.

Status of Clinical Training at Tokyo Dental College and Subsequent Career Pathway - Focusing on Training Facilities and Programs.

The goal of the present study was to clarify the status of training in clinical dentistry at training facilities belonging to Tokyo Dental College by investigating trainee quota fill rates and analyzing the relationship between selection of training program and subsequent career pathway. The trainees were divided into 4 groups based on career pathway after training: those entering graduate school; those becoming residents; those remaining at the college or hospital to take specialized clinical courses or for other purposes; and those starting work outside the college. The trainee quota fill rate was highest at the Suidobashi Hospital, followed by the Ichikawa General and Chiba Hospitals. A total of 8 training programs have been implemented at these facilities (Chiba: A, B, C, and D; Suidobashi, A, B, and C; and Ichikawa, A). A comparison among training programs revealed that the fill rate exceeded 60% in all cases, except for Chiba D, where it was markedly lower, at 25%. Career pathway after training significantly varied between training facilities. Among trainees selecting the Chiba Hospital, the highest proportion was occupied by those starting work outside the college. The proportion of those entering graduate school was also high in this group. Similarly, among trainees selecting the Suidobashi Hospital, the highest proportion was occupied by those starting work outside the college. The proportion of those entering graduate school was low in this group, however. Among trainees selecting the Ichikawa General Hospital, the proportions of those entering graduate school and those becoming residents were particularly high, while the proportion of those starting work outside the college was low. A comparison of career pathways revealed a significant difference only between Chiba Programs B and C. In conclusion, training facility showed a markedly stronger influence on career pathway than training program.

Basic Finite Element Analysis of Para-periodontal Ligament in All-ceramic Zirconia Fixed Partial Denture.

The purpose of the present study was to investigate the validity of incorporating a para-periodontal ligament in the test mold used in a basic fracture test of a zirconia all-ceramic fixed partial denture (FPD). A simplified three-dimensional finite element analysis model was designed based on the three-unit FPD fracture test. Two types of model, one with and one without a para-periodontal ligament between the abutment and base mold, were fabricated. Microfocus CT of the missing first molar area in a dry human mandible was performed. A three-dimensional model was then fabricated based on the data obtained. A load of 600 N was applied to the center of the pontic and stress distribution observed. The model with the para-periodontal ligament showed stress dispersion to the dental root with rotation of the abutment mold. Stress distribution in the finite element analysis model with a para-periodontal ligament showed greater similarity with that in the mandibular model than with that in the other two models without a para-periodontal ligament.

Influence of plasma and ultraviolet treatment of zirconia on initial attachment of human oral keratinocytes: expressions of laminin γ2 and integrin ß4.

Initial attachment of human oral keratinocytes cultured on yttria-stabilized tetragonal zirconia polycrystal (TZP) surfaces that were subjected to UV or oxygen plasma (O2-plasma) treatment was investigated. The viability of the attached cells, mRNA expression of laminin γ2 and integrin ß4, distribution of laminin γ2 and integrin ß4, cell area, and cell morphology were assessed. The results showed that no differences in the viability of attached cells were recognized among the conditions. However, expression of laminin γ2 and integrin ß4 as well as cell morphology were promoted only in O2-plasma specimens even though superhydrophilicity was obtained in both the UV and O2-plasma specimens compared with the untreated control specimen. The photocatalytic activity was believed to be closely involved in the above-mentioned differences. The results of this study suggest that TZP surface treated with oxygen plasma promotes the initial attachment capability of human oral keratinocytes with enhancing the extracellular matrix such as laminin γ2.

Effect of connector design on fracture resistance of zirconia all-ceramic fixed partial dentures.

The purpose of the present study was to determine the relationship between cross-sectional design and fracture load using a static load bearing test in yttria-stabilized tetragonal zirconia polycrystal ceramic frameworks on a molar fixed partial denture. The test framework was designed as a 3-unit bridge with two abutment teeth at the second premolar and second molar of the mandible. The cross-sectional area of the connector was 9.0, 7.0, or 5.0mm(2). In terms of shape, the cross-section was either circular or oval, with a height/width ratio of 1:1, 3:4, or 2:3. For each of the 9 combinations of cross-sectional area and shape, 5 frameworks were prepared (45 in total). Frameworks were cemented to a metallic test model with adhesive resin cement. After fracture load was measured, the percentage of fracture sites was determined and the fracture surfaces observed. In terms of cross-sectional area, there was a statistically significant difference in fracture load between 9.0, 7.0, and 5.0mm(2). No significant difference in fracture load was observed between any two shapes of connector (p>0.05). The fracture load of all frameworks with a cross-sectional area of 9.0 or 7.0mm(2) was over 880 N, which was recognized as parafunctional occlusal force. Fracture occurred at the distal connector in 82.2% of all frameworks on average. Fracture load decreased as cross-sectional area of the connector became smaller. The cross-sectional shape used in the present study was less influential on fracture load. It appears to be clinical possible to apply a connector with a cross-sectional area of 7.0mm(2). Fracture often occurred at the distal connector between the pontic and the abutment, corresponding to the second molar.

Electromyographic response in inferior head of human lateral pterygoid muscle to anteroposterior postural change during opening and closing of mouth.

The purpose of this study was to investigate the influence of anteroposterior postural change on electromyography (EMG) activity in the lateral pterygoid muscle. Subjects consisted of 7 patients attending this hospital for close examination. The inferior heads of the lateral pterygoid and masseter muscles were chosen as evaluation sites. For the EMG recordings, the test movement was opening and closing of the mouth; postural conditions were the upright and supine positions. The mean value of EMG activity in the inferior head of the lateral pterygoid muscle was calculated. During mouth-opening in 5 out of the 7 patients, and during mouth-closing in 2 out of the 7 patients, mean value of EMG activity differed significantly with body position. Mean value of EMG activity was reduced in the supine position. The results revealed that anteroposterior postural change affected mean value of EMG activity in this muscle.